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SAS: Split Antibiotic Selection for identifying chaperones that improve protein solubility

2023-08-11

Abstract excerpt

<h4>Background: </h4> Heterologous expression of active, native-folded protein in Escherichia coli is critical in both academic research and biotechnology settings. When expressing non-native recombinant proteins in E. coli , obtaining soluble and active protein can be challenging. Numerous techniques can be used to enhance a proteins solubility, and largely focus on either altering the expression strain, plasmid...

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Literature Corpus work
78914bfd-10ce-5dda-be25-8f389151e0d4
DOI
10.21203/rs.3.rs-3239197/v1
Open publication

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SAS: Split Antibiotic Selection for identifying chaperones that improve protein solubilityDOI 10.21203/rs.3.rs-3239197/v1
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