An Efficient Vector-based CRISPR/Cas9 System in an <i>Oreochromis mossambicus</i> Cell Line using Endogenous Promoters

Abstract excerpt

CRISPR/Cas9 gene editing is effective in manipulating genetic loci in mammalian cell cultures and whole fish but efficient platforms applicable to fish cell lines are currently limited. Our initial attempts to employ this technology in fish cell lines using heterologous promoters or a ribonucleoprotein approach failed to indicate genomic alteration at targeted sites in a tilapia brain cell line (OmB). For potentia...

Topics

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• CRISPR-Cas Systems
• Gene Editing
• Gene Expression Regulation, Fungal
• Gene Knockdown Techniques
• Genome-Wide Association Study
• Mutation
• Schizosaccharomyces
• Transcription, Genetic

Identifiers and source

Literature Corpus work

78690e1d-e3e9-5707-82b3-62569ac70320

DOI

10.1101/2020.08.04.237065