Article

Direct RNA sequencing of Respiratory Syncytial Virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundance

2021-12-23

Abstract excerpt

To characterize species of viral mRNA transcripts generated during respiratory syncytial virus (RSV) infection, human fibroblast-like MRC5 lung cells were infected with subgroup A RSV for 6, 16 and 24 hours. Total RNA was harvested and polyadenylated mRNA was enriched and sequenced by direct RNA sequencing on an Oxford nanopore device. This yielded over 150,000 direct mRNA transcript reads which were mapped to the...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 721d8fa0-e180-5a73-96be-6775ad0d65b2
DOI: 10.1101/2021.12.23.473996

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Direct RNA sequencing of Respiratory Syncytial Virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundanceDOI 10.1101/2021.12.23.473996Read

Direct RNA sequencing of respiratory syncytial virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundanceSemantic proximity Read

Long-read RNA sequencing identifies polyadenylation elongation and differential transcript usage of host transcripts during SARS-CoV-2 <i>in vitro</i> infectionSemantic proximity Read

Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infectionSemantic proximity Read

Unravelling the complexities of respiratory syncytial virus RNA synthesisSemantic proximity Read

Long-read sequencing of SARS-CoV-2 reveals novel transcripts and a diverse complex transcriptome landscapeSemantic proximity Read

Combined Nanopore and Single-Molecule Real-Time Sequencing Survey of Human Betaherpesvirus 5 TranscriptomeSemantic proximity Read

Viral Hijacking of Host Polyadenylation Machinery to Regulate Post-Transcriptional Gene ExpressionSemantic proximity Read

Genes with 5′ terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 proteinSemantic proximity Read

Viral and host small RNA transcriptome analysis of SARS-CoV-1 and SARS-CoV-2-infected human cells reveals novel viral short RNAsSemantic proximity Read

Viral N6-methyladenosine upregulates replication and pathogenesis of human respiratory syncytial virusSemantic proximity Read