Article

Multifunctional CRISPR/Cas9 with engineered immunosilenced human T cell epitopes

2018-07-02

Abstract excerpt

The application of Cas9 for genetic and epigenetic therapies in humans raises concerns over immunogenicity of this foreign protein. We report pre-existing human CD8+ T cell immunity to Streptococcus pyogenes Cas9 in the majority of healthy individuals screened. In a proof-of-principle study, we demonstrate that Cas9 protein can be modified to eliminate immunodominant epitopes through targeted mutation while prese...

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Literature Corpus work: 71d9fad9-8722-5b7a-90df-a2e75cdba66b
DOI: 10.1101/360198

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