Article

Analytical Assessment of Metagenomic Workflows for Pathogen Detection with NIST RM 8376 and Two Sample Matrices

2024-09-26

Abstract excerpt

We assessed the analytical performance of metagenomic workflows using NIST Reference Material 8376 DNA from bacterial pathogens spiked into two simulated clinical samples: cerebral spinal fluid (CSF) and stool. Sequencing and taxonomic classification were used to generate signals for each sample and taxa of interest, and to estimate the limit of detection (LOD), the response function, and linear dynamic range. We...

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Literature Corpus work: 712ac44e-5bc8-52e8-8102-43345a1bf44f
DOI: 10.1101/2024.09.24.614717

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