Article

Production of human translation-competent lysates using dual centrifugation

2021-05-27

Abstract excerpt

<h4>ABSTRACT</h4> Protein synthesis is a central process in gene expression and the development of efficient in vitro translation systems has been the focus of scientific efforts for decades. The production of translation-competent lysates originating from human cells or tissues remains challenging, mainly due to the variability of cell lysis conditions. Here we present a robust and fast method based on dual cen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6c032429-060e-5de3-9a51-387fb7d2d95a
DOI: 10.1101/2021.05.27.445927

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Production of human translation-competent lysates using dual centrifugationDOI 10.1101/2021.05.27.445927Read

Efficient cell-free translation from diverse human cell typesSemantic proximity Read

A nanobody-based strategy for rapid and scalable purification of native human protein complexesSemantic proximity Read

Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid ExtractionSemantic proximity Read

Overcoming the eIF2α Brake in Human Cell-Derived Translation SystemsSemantic proximity Read

A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studiesSemantic proximity Read

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditionsSemantic proximity Read

Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EMSemantic proximity Read

Transcriptome analysis of EPO and GFP HEK293 Cell-lines Reveal Shifts in Energy and ER Capacity Support Improved Erythropoietin Production in HEK293F CellsSemantic proximity Read

Selectable high‐yield recombinant protein production in human cells using a GFP/YFP nanobody affinity supportSemantic proximity Read

Identification and molecular characterization of process relevant microRNAs in Chinese Hamster Ovary (CHO) cells : CHO optimization for the production of recombinant proteins of pharmaceutical interestSemantic proximity Read

Protein-free media for cardiac differentiation of hPSCs in 2000 mL suspension cultureSemantic proximity Read

Redesigned upstream processing enables a 24-hour workflow from <i>E. coli</i> cells to cell-free protein synthesisSemantic proximity Read