Back to search

Article

Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems

2025-11-16

Abstract excerpt

Cell-free translation from human cells is a powerful platform for studying mammalian gene expression and building synthetic biology tools, but productivity is often curtailed by inhibitory phosphorylation of eIF2α on residue Ser52. Here we systematically explored complementary strategies to bypass this initiation block across editable and hard-to-edit human cell types. In Expi293F suspension cells, precise genome...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
d06c60f8-457a-50c5-bb91-4de84f5b8b7f
DOI
10.1101/2025.11.16.688697
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 8 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Overcoming the eIF2α Brake in Human Cell-Derived Translation SystemsDOI 10.1101/2025.11.16.688697
Efficient cell-free translation from diverse human cell typesSemantic proximity
Production of human translation-competent lysates using dual centrifugationSemantic proximity
Residues required for phosphorylation of translation initiation factor eIF2α under diverse stress conditions are divergent between yeast and human.Semantic proximity
Pervasive translation of the downstream ORF from bicistronic mRNAs by human cells: impact of the upstream ORF codon usage and splicingSemantic proximity
Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo.Semantic proximity
Paradoxical Sensitivity to an Integrated Stress Response Blocking Mutation in Vanishing White Matter Cells.Semantic proximity
Splice-Aware Optimization Prevents Pervasive Missplicing of Natural and Synthetic cDNAsSemantic proximity
Generation of gene-corrected human isogenic iPSC lines from hypertrophic cardiomyopathy patients harboring PRKAG2 mutation (c.2084A>G, p.His530Arg) using prime editing.Semantic proximity
Select a neighboring publication to make it the new centre.