Article

Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples

2024-06-20

Abstract excerpt

<h4>Background</h4> Currently, diagnosis of bacterial infections is based on culture, possibly followed by the amplification and sequencing (Sanger method) of the 16S rDNA - encoding gene when cultures are negative. Clinical metagenomics (CMg), i.e. the sequencing of a sample’s entire nucleic acids, may allow for the identification of bacteria not detected by conventional methods. Here, we tested the performance o...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6b23e593-e91f-571d-8c39-fce509bf0b80
DOI: 10.1101/2024.06.18.24309080

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samplesDOI 10.1101/2024.06.18.24309080Read

Clinical Efficacy and Diagnostic Value of Metagenomic Next- Generation Sequencing for Rapid Pathogen Detection in Patients with Suspected Infectious DiseasesSemantic proximity Read

Metagenomic sequencing as a clinical diagnostic tool for infectious diseases: a systematic review and meta-analysisSemantic proximity Read

Detection of bacterial pathogens from clinical specimens using conventional microbial culture and 16S metagenomics: a comparative studySemantic proximity Read

Prospective Comparison Between Shotgun Metagenomics and Sanger Sequencing of the 16S rRNA Gene for the Etiological Diagnosis of InfectionsSemantic proximity Read

Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative InfectionsSemantic proximity Read

Clinical metagenomics of bone and joint infections: a proof of concept studySemantic proximity Read

The Role of Metagenomics and Next-Generation Sequencing in Infectious Disease DiagnosisSemantic proximity Read

Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic laboratorySemantic proximity Read

Metagenomic analysis of bacterial infections by means of high-throughput DNA sequencingSemantic proximity Read

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious DiseasesSemantic proximity Read

Metagenomic Sequencing Enables Clinically Relevant Identification of 176 Targets from Faecal SamplesSemantic proximity Read

Point-Counterpoint: Should We Be Performing Metagenomic Next-Generation Sequencing for Infectious Disease Diagnosis in the Clinical Laboratory?Semantic proximity Read