Prospective Comparison Between Shotgun Metagenomics and Sanger Sequencing of the 16S rRNA Gene for the Etiological Diagnosis of Infections

Abstract excerpt

Bacteriological diagnosis is traditionally based on culture. However, this method may be limited by the difficulty of cultivating certain species or by prior exposure to antibiotics, which justifies the resort to molecular methods, such as Sanger sequencing of the 16S rRNA gene (Sanger 16S). Recently, shotgun metagenomics (SMg) has emerged as a powerful tool to identify a wide range of pathogenic microorganisms...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacterial Identification and Susceptibility Testing
• Share to Molecular Biology Techniques and Applications
• Share to Mycobacterium research and diagnosis