Article

High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations

2026-07-30

Abstract excerpt

Two-photon calcium imaging is a standard tool for measuring neuronal population activity in vivo, yet how axial resolution, sensor expression strategy, and analysis pipeline jointly affect data accuracy remains poorly characterized. Here, we imaged the same L2/3 neurons in mouse primary visual cortex at five axial resolutions (3.6 - 21.0 μm), spanning current two-photon systems from benchtop microscopes to large-f...

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Literature Corpus work: 671ad943-3017-54ed-a884-889965ec2f63
DOI: 10.64898/2026.07.28.741086

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