Article
Improved isolation of murine hepatocytes for in vitro malaria liver stage studies
1 Dec 2007
Abstract excerpt
BACKGROUND: Primary hepatocyte cultures are a valuable tool for the understanding of cellular and molecular phenomena occurring during malaria liver stage. This paper describes an improved perfusion/dissociation procedure to isolate hepatocytes from mouse liver that is suitable for malaria studies and allows reproducible preparation of primary hepatocytes with consistent cell yields and controlled purity....
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