Article

Spatial transcriptomics of tumor microenvironment in formalin-fixed paraffin-embedded breast cancer

2020-02-02

Abstract excerpt

Tumor samples are conserved in clinical practice in formalin-fixed paraffin-embedded (FFPE) blocks. Formalin fixation chemically alters nucleic acids, rendering transcriptomic analysis challenging. RNA-sequencing is usually performed on tumor bulk, without distinction of cell subtypes or location. Here we describe the development of a robust method for RNA extraction and exome-capture RNA-sequencing of laser-captu...

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Literature Corpus work: 65895b5f-74cb-542f-9ed6-5882d32cf1c6
DOI: 10.1101/2020.01.31.928143

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