Back to search

Article

Evaluation of DNA extraction methods for clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing

2025-11-18

Abstract excerpt

<title>Abstract</title> <p> Whole-genome sequencing (WGS) has potential for determining the complete drug-resistance profile of clinical <italic>Mycobacterium tuberculosis</italic> ( <italic>Mtb</italic> ) strains. Cetyltrimethylammonium-bromide (CTAB), the conventional method for <italic>Mtb</italic> DNA extraction, is labour-intensive and difficult to implement in routine laboratory settings. This study...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
59d0e3d9-9e80-59d3-b093-78fa7b815346
DOI
10.21203/rs.3.rs-7905849/v1
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Evaluation of DNA extraction methods for clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencingDOI 10.21203/rs.3.rs-7905849/v1
A mycobacterial DNA extraction protocol designed for resource limited settings generates high quality whole genome sequencingSemantic proximity
Removing the bottleneck in whole genome sequencing of Mycobacterium tuberculosis for rapid drug resistance analysis: a call to actionSemantic proximity
Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) CulturesSemantic proximity
Systematic review and meta-analysis of protocols and sequencing yield for whole genome sequencing of Mycobacterium tuberculosis directly from sputum samples.Semantic proximity
A universal, high-quality, and high-yield DNA purification method for mycobacteria, including Mycobacterium tuberculosis : large-scale assessment of the chloroform-bead methodSemantic proximity
Genomic Sequencing from Sputum for Tuberculosis Disease Diagnosis, Lineage Determination and Drug Susceptibility PredictionSemantic proximity
A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cellsSemantic proximity
Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome SequencingSemantic proximity
Protocol for DNA Extraction from QuantiFERON-TB Gold Tubes for PCR and Sequencing ApplicationsSemantic proximity
Same-Day Diagnostic and Surveillance Data for Tuberculosis via Whole-Genome Sequencing of Direct Respiratory SamplesSemantic proximity
Same-day diagnostic and surveillance data for tuberculosis via whole genome sequencing of direct respiratory samplesSemantic proximity
Initial experiences with <i>Mycobacterium tuberculosis</i> DNA extraction for downstream Deeplex Myc-TB targeted deep sequencing in a high burden settingSemantic proximity
Select a neighboring publication to make it the new centre.