Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) Cultures

29 Jan 2015

Abstract excerpt

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA extraction method is based on ethanol precipitation supplemented by pretreatment steps with a MolYsis kit or saline...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Infectious Diseases and Mycology
Share to Mycobacterium research and diagnosis
Share to Tuberculosis Research and Epidemiology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 25631807
DOI: 10.1128/jcm.03073-14

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) CulturesPMID 25631807Read ↗

DNA Extraction Method Optimized for Nontuberculous Mycobacteria Long-Read Whole Genome SequencingSemantic proximity Read ↗

A mycobacterial DNA extraction protocol designed for resource limited settings generates high quality whole genome sequencingSemantic proximity Read ↗

A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cellsSemantic proximity Read ↗

Complete nontuberculous mycobacteria whole genomes using an optimized DNA extraction protocol for long-read sequencingSemantic proximity Read ↗

Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome SequencingSemantic proximity Read ↗

Evaluation of DNA extraction methods for clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencingSemantic proximity Read ↗

A universal, high-quality, and high-yield DNA purification method for mycobacteria, including Mycobacterium tuberculosis : large-scale assessment of the chloroform-bead methodSemantic proximity Read ↗

Same-day diagnostic and surveillance data for tuberculosis via whole genome sequencing of direct respiratory samplesSemantic proximity Read ↗

Same-Day Diagnostic and Surveillance Data for Tuberculosis via Whole-Genome Sequencing of Direct Respiratory SamplesSemantic proximity Read ↗

Optimizing mycobacteria molecular diagnostics: No decontamination! Human DNA depletion? Greener storage at 4 °C!Semantic proximity Read ↗

Protocol for DNA Extraction from QuantiFERON-TB Gold Tubes for PCR and Sequencing ApplicationsSemantic proximity Read ↗

Systematic review and meta-analysis of protocols and sequencing yield for whole genome sequencing of Mycobacterium tuberculosis directly from sputum samples.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.