Article

3D Quantification of Viral Transduction Efficiency in Living Human Retinal Organoids

2024-03-12

Abstract excerpt

The development of therapeutics builds on testing their efficiency in vitro. To optimize gene therapies, for example, fluorescent reporters expressed by treated cells are typically utilized as readouts. Traditionally, their global fluorescence signal has been used as an estimate of transduction efficiency. However, analysis in individual cells within a living 3D tissue remains a challenge. Readout on a single-cell...

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Literature Corpus work: 578871c0-58a6-5b64-9efd-b2cfd3057996
DOI: 10.1101/2024.03.06.583795

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