Article

Evaluating primer and probe mismatch tolerance in an Influenza A matrix gene RT-qPCR using contemporary human and zoonotic strains

2026-02-23

Abstract excerpt

<h4>Background</h4> Genetic drift and host-associated adaptation in influenza A viruses threaten the long-term reliability of RT-qPCR–based diagnostics, particularly when nucleotide mismatches arise within primer and probe binding regions. Conventional assay evaluations often emphasize sequence conservation but rarely assess functional mismatch tolerance across divergent subtypes and hosts. <h4>Methods</h4> We p...

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Literature Corpus work: 5315d0ba-0b00-5880-bbaa-2719055289f2
DOI: 10.64898/2026.02.23.707407

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