Article

Development of human skin equivalents with inducible ceramide depletion for in vitro modelling of lipid deficiency

2025-01-24

Abstract excerpt

The lipid composition of the epidermis plays a critical role in the skin’s barrier function, and defects in lipid synthesis or assembly can cause a spectrum of skin diseases, ranging from dry skin to severe ichthyoses. The aim of this study was to develop an in vitro model of human skin with tuneable lipid deficiency. Human N/TERT keratinocytes were engineered to express doxycycline-inducible short hairpin RNAs ta...

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Literature Corpus work: 4f08872a-e95b-564e-b9ad-793aec5bf63c
DOI: 10.1101/2025.01.20.633956

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