Article

Managing Autofluorescence in Spectral Flow Cytometry for Macrophage Identification in the Liver

2026-01-31

Abstract excerpt

Autofluorescence (AF) in biological tissues arises from the natural emission of light by intra- and extracellular molecules upon light absorption. Conventional flow cytometry cannot correct for cellular AF, leading to distorted signals and measurement errors. While spectral flow cytometry enables AF visualization and extraction, accurately correcting for AF remains challenging in complex biological samples contain...

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Literature Corpus work: 3d7d6c77-71f8-5ced-8fbf-2e8721ba4d5c
DOI: 10.64898/2026.01.28.702253

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