Article

Inhibition of Polymerase Chain Reaction: Pathogen-Specific Controls are better than Human Gene Amplification

2019-06-24

Abstract excerpt

PCR inhibition is frequent in medical microbiology routine practice and may lead to false-negative results; however there is no consensus on how to detect it. Pathogen-specific and human gene amplifications are widely used to detect PCR inhibition. We aimed at comparing the value of PCR inhibitor detection using these two methods. We analysed Cp shifts (ΔCp) obtained from qPCRs targeting either the albumin gene or...

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Literature Corpus work: 3c331758-5bf2-544f-87ae-831500094581
DOI: 10.1101/681585

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