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Optimizing bacterial DNA extraction in urine

24 Sept 2019

Abstract excerpt

Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections. However, low quantities of bacterial DNA and PCR inhibitors in urine may prevent efficient PCR amplification for molecular detection of bacteria. Furthermore, cold temperatures used to preserve DNA and bacteria in urine can promote precipitation of crystals that...

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PMID: 31550285
DOI: 10.1371/journal.pone.0222962

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