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Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?

11 Jun 2004

Abstract excerpt

We applied real-time broad-range polymerase chain reaction (PCR) to detect bacteraemia in blood from febrile patients. Interpretation of amplification results in relation to clinical data and blood culture outcome was complex, although the reproducibility of the PCR results was good. Sequencing analysis of the PCR products revealed the presence of Burkholderia species DNA while no Burkholderia species grew in...

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PMID: 15364111
DOI: 10.1016/j.femsim.2004.05.009

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