Detection of Periprosthetic Infections With Use of Ribosomal RNA-Based Polymerase Chain Reaction

Abstract excerpt

BACKGROUND: Previously described molecular biology techniques used to detect periprosthetic infections have been complicated by false-positive results. We have reported the development of a messenger RNA (mRNA)-based procedure to reduce these false-positive results. The limitations of this procedure are the lack of a universal target and reduced sensitivity due to a low concentration of bacterial mRNAs in test...

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