Article

A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader

2021-12-10

Abstract excerpt

<h4>ABSTRACT</h4> We describe a generalizable time-resolved Förster resonance energy transfer (TR-FRET)-based platform to profile the cellular action of heterobifunctional degraders (or proteolysis-targeting chimeras; PROTACs), capable of both accurately quantifying protein levels in whole cell lysates in less than 1 h and measuring small-molecule target engagement to en-dogenous proteins, here specifically for h...

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Literature Corpus work: 39717efd-e9ca-5a8a-a56e-7e4a2a892b6c
DOI: 10.1101/2021.12.08.471806

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