Direct PCR offers a fast and reliable alternative to conventional DNA isolation methods for animal gut microbiomes

Abstract excerpt

The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures, specifically the isolation of DNA and generation of amplicon libraries. Recently, direct PCR kits have been developed that circumvent conventional DNA extraction steps, thereby s...

Topics

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• Clostridium difficile and Clostridium perfringens research
• Colorectal Cancer Screening and Detection
• Diet and metabolism studies
• Dietary Effects on Health
• Dysphagia Assessment and Management
• Fecal contamination and water quality
• Food Security and Health in Diverse Populations
• Gut microbiota and health

Identifiers and source

Literature Corpus work

38484c9b-3a4f-5d4f-bdc0-20ce274a4a9b

DOI

10.1101/193664