Article

A stem cell knockout village reveals lineage rewiring and a non-canonical islet cell fate in monogenic diabetes

2025-12-25

Abstract excerpt

Genetics studies have identified a core set of regulators essential for pancreatic β cell development, many of which are mutated in monogenic diabetes. However, how these mutations alter developmental trajectories to produce pathological cell states remains elusive. Here we introduce a knockout village framework that enables longitudinal scRNA-seq profiling of 79 human pluripotent stem cell mutant lines targeting...

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Literature Corpus work: 2f0e04b6-3d6e-578c-b9a9-0c289475b6d1
DOI: 10.64898/2025.12.23.696311

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