Article

Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflows

2025-07-15

Abstract excerpt

<h4>SUMMARY</h4> Activation-induced marker (AIM) assays are a promising tool to track antigen-specific T cells, but methodological heterogeneity between research groups hinders their clinical utility. To evaluate AIM assay reproducibility, we conducted a multi-site study of SARS-CoV-2 and cytomegalovirus AIMs. We found inherent variability in AIM assays and optimized approaches to enhance reproducibility, includi...

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Literature Corpus work: 2c9e6394-8796-54c4-88ea-2a29792bef4e
DOI: 10.1101/2025.07.15.664847

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