Article

Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

2020-07-22

Abstract excerpt

The Cre/ lox recombinase system has been widely used for spatiotemporal control of gene expression in animal model systems, however, efficient methods to isolate zebrafish Cre drivers that reliably recapitulate endogenous gene expression patterns are needed. Here, we apply CRISPR/Cas9 targeting to integrate a 2A-Cre recombinase transgene with 48bp homology arms into proneural genes ascl1b , olig2 and neurod1 ....

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Literature Corpus work: 2b1ec6e8-5eda-559f-a3f5-89283e8c3430
DOI: 10.1101/2020.07.21.214452

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