Article

DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing

2022-03-01

Abstract excerpt

Long single-molecular sequencing, such as PacBio circular consensus sequencing (CCS) and nanopore sequencing, is advantageous in detecting DNA 5-methylcytosine (5mC) in CpGs, especially in repetitive genomic regions. However, existing methods for detecting 5mCpGs using PacBio CCS are less accurate and robust. Here, we present ccsmeth, a deep-learning method to detect DNA 5mCpGs using CCS reads. We sequence PCR-tre...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 296b37c9-48bd-5584-8699-3d49e673aa7b
DOI: 10.1101/2022.02.26.482074

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencingDOI 10.1101/2022.02.26.482074Read

Whole human genome 5’-mC methylation analysis using long read nanopore sequencingSemantic proximity Read

A new approach to decode DNA methylome and genomic variants simultaneously from double strand bisulfite sequencing.Semantic proximity Read

Fine Mapping Regulatory Variants by Characterizing Native CpG Methylation with Nanopore Long-Read SequencingSemantic proximity Read

Genome-wide methylation detection and episignature analysis using PacBio long-read sequencingSemantic proximity Read

NANOME: A Nextflow pipeline for haplotype-aware allele-specific consensus DNA methylation detection by nanopore long-read sequencingSemantic proximity Read

MethPhaser: methylation-based long-read haplotype phasing of human genomes.Semantic proximity Read

DNA methylation calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluationSemantic proximity Read

Comparing methylation levels assayed in GC-rich regions with current and emerging methodsSemantic proximity Read

Phasing DNA Methylation.Semantic proximity Read

MethPhaser: methylation-based haplotype phasing of human genomesSemantic proximity Read

Nanopore sequencing and assembly of a human genome with ultra-long readsSemantic proximity Read

Methylation-aware long-read phasing significantly improves genome-wide haplotype reconstructionSemantic proximity Read