Article
Identification of Vaccine-Altered Circulating B Cell Phenotypes Using Mass Cytometry and a Two-Step Clustering Analysis.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jun 2016
Pejoski David, Tchitchek Nicolas, Rodriguez Pozo André, Elhmouzi-Younes Jamila, Yousfi-Bogniaho Rahima, Rogez-Kreuz Christine, Clayette Pascal, Dereuddre-Bosquet Nathalie, Lévy Yves, Cosma Antonio, Le Grand Roger, Beignon Anne-Sophie
Abstract excerpt
Broadening our understanding of the abundance and phenotype of B cell subsets that are induced or perturbed by exogenous Ags will improve the vaccine evaluation process. Mass cytometry (CyTOF) is being used to increase the number of markers that can be investigated in single cells, and therefore characterize cell phenotype at an unprecedented level. We designed a panel of CyTOF Abs to compare the B cell response...
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