Article

Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

2013-01-01

Abstract excerpt

RNA-Seq is an effective method to study the transcriptome, but can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations, or cadavers. Recent studies have proposed several methods for RNA-Seq of low quality and/or low quantity samples, but their relative merits have not been systematically analyzed. Here, we compare five such methods using metrics relevant to transcript...

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Literature Corpus work: 247dd5e2-f597-5d28-ac38-43e9199a2ec6
DOI: 10.1038/nmeth.2483

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