Article

Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

2020-06-04

Abstract excerpt

We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome profiling. FAIMS effectively filtered out singly charged ions for more effective MS analysis of multiply charged peptides, resulting in an average of 1056 protein...

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Identifiers and source

Literature Corpus work: 4b021ec0-e384-5360-b496-79d017e07916
DOI: 10.1101/2020.06.03.132449

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