Article

A resource and computational approach for quantifying gene editing allelism at single-cell resolution

2025-05-27

Abstract excerpt

CRISPR-Cas9-based gene editing is a powerful approach to developing gene and cell therapies for several diseases. Engineering cell therapies requires accurate assessment of gene editing allelism because editing patterns can vary across cells leading to genotypic heterogeneity. This can hinder development of robust cell therapies. Droplet-based targeted single-cell DNA sequencing (scDNAseq) has been used to genotyp...

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Literature Corpus work: 227a060a-04f5-51e4-a65d-be71554db2d5
DOI: 10.1101/2025.05.22.653824

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