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Article

Multiplexed droplet single-cell RNA-sequencing using natural genetic variation.

Nature biotechnology - 1 Jan 2018

Kang Hyun Min, Subramaniam Meena, Targ Sasha, Nguyen Michelle, Maliskova Lenka, McCarthy Elizabeth, Wan Eunice, Wong Simon, Byrnes Lauren, Lanata Cristina M, Gate Rachel E, Mostafavi Sara, Marson Alexander, Zaitlen Noah, Criswell Lindsey A, Ye Chun Jimmie

Abstract excerpt

Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that harnesses natural genetic variation to determine the sample identity of each droplet containing...

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Identifiers

PMID: 29227470
DOI: 10.1038/nbt.4042
PMCID: PMC5784859

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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