Article
Souporcell: robust clustering of single-cell RNA-seq data by genotype without reference genotypes.
Nature methods - 1 Jun 2020
Heaton Haynes, Talman Arthur M, Knights Andrew, Imaz Maria, Gaffney Daniel J, Durbin Richard, Hemberg Martin, Lawniczak Mara K N
Abstract excerpt
Methods to deconvolve single-cell RNA-sequencing (scRNA-seq) data are necessary for samples containing a mixture of genotypes, whether they are natural or experimentally combined. Multiplexing across donors is a popular experimental design that can avoid batch effects, reduce costs and improve doublet detection. By using variants detected in scRNA-seq reads, it is possible to assign cells to their donor of origin...
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