Article

Affinity maturation of anti-NT-proBNP single-chain variable fragment (scFv) and biophysical characterization for heart failure biosensor application

2025-09-16

Abstract excerpt

Rapid, specific detection of N-terminal pro-B-type natriuretic peptide (NT-proBNP) enhances heart-failure diagnosis. An NT-proBNP–binding single-chain variable fragment (scFv) bearing a gold-assembling linker (C12AP) was engineered and affinity-matured to serve as a biosensor recognition element. Docking of C12AP–NT-proBNP complexes guided semi-rational mutagenesis at D110 (VH-CDR3) and Y174 (VL-CDR1). Twenty-two...

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Literature Corpus work: 1ea1066c-3e76-56e5-b4ec-6822934d11c9
DOI: 10.22541/au.175802243.32591328/v1

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