Article

Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants

2019-02-23

Abstract excerpt

Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR (TKC) technology that uses...

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Literature Corpus work: 1c38d824-67aa-56d4-9e5a-2fbd99f3377d
DOI: 10.1016/j.rsci.2018.11.001

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