Article

A SARS-CoV-2 mini-genome assay based on negative-sense RNA to study replication inhibitors and emerging mutations

2021-06-28

Abstract excerpt

Severe Acute Respiratory Coronavirus 2 (SARS-CoV-2) is a positive-sense single-stranded RNA virus and the causative agent of the Coronavirus disease 2019 (COVID-19) pandemic. Efforts to identify inhibitors of SARS-CoV-2 replication enzymes and better understand the mechanisms underlying viral RNA synthesis have largely relied on biosafety level 3 (BSL3) laboratories, limiting throughput and accessibility. Recently...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1665b91f-bbaf-5b97-ab7a-a1047505c1c4
DOI: 10.1101/2021.06.28.450211

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A SARS-CoV-2 mini-genome assay based on negative-sense RNA to study replication inhibitors and emerging mutationsDOI 10.1101/2021.06.28.450211Read

Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and ReplicationSemantic proximity Read

Subgenomic SARS-CoV-2 replicon and reporter replicon cell lines enable ultrahigh throughput antiviral screening and mechanistic studies with antivirals, viral mutations or host factors that affect COVID-19 replicationSemantic proximity Read

Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testingSemantic proximity Read

Construction and characterization of two SARS‐CoV‐2 minigenome replicon systemsSemantic proximity Read

Engineering a Reliable and Convenient SARS-CoV-2 Replicon System for Analysis of Viral RNA Synthesis and Screening of Antiviral Inhibitors.Semantic proximity Read

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12aSemantic proximity Read

A luminescent attenuated SARS-CoV-2 for the identification and validation of drug-resistant mutants.Semantic proximity Read

Development, applications, and future prospects of RNA virus reverse genetics technologySemantic proximity Read

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12aSemantic proximity Read

Reverse genetics systems for SARS‐CoV‐2Semantic proximity Read

Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reactionSemantic proximity Read

Construction of a Noninfectious SARS-CoV-2 Replicon for Antiviral-Drug Testing and Gene Function StudiesSemantic proximity Read