Article

Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus

2018-07-26

Abstract excerpt

Propagation of human cytomegalovirus (CMV) in cultured cells results in genetic adaptations that confer improved growth in vitro and significant attenuation in vivo. Mutations in RL13 arise quickly during cell culture passage, while mutations in the UL128-131A locus emerge later during fibroblast passage and disrupt expression of a glycoprotein complex that is important for entry into epithelial and endothelial ce...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 15f6ff1c-a283-5e3c-be00-baeb549753e6
DOI: 10.20944/preprints201807.0503.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Click a neighbor to travelStep 1 · 12 closest

Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human CytomegalovirusDOI 10.20944/preprints201807.0503.v1Read

Repair of a Mutation Disrupting the Guinea Pig Cytomegalovirus Pentameric Complex Acquired during Fibroblast Passage Restores Pathogenesis in Immune-Suppressed Guinea Pigs and in the Context of Congenital Infection.Semantic proximity Read

Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture.Semantic proximity Read

Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.Semantic proximity Read

The susceptibility of primary cultured rhesus macaque kidney epithelial cells to rhesus cytomegalovirus strainsSemantic proximity Read

The pentameric complex drives immunologically covert cell-cell transmission of wild-type human cytomegalovirus.Semantic proximity Read

Coding potential of UL/b' from the initial source of rhesus cytomegalovirus Strain 68-1.Semantic proximity Read

The pentameric complex is not required for vertical transmission of cytomegalovirus in seronegative pregnant rhesus macaquesSemantic proximity Read

A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig CytomegalovirusSemantic proximity Read

Cytomegalovirus Strain TB40/E Restrictions and Adaptations to Growth in ARPE-19 Epithelial CellsSemantic proximity Read

A Comparison between Low- and High-Passage Strains of Human Cytomegalovirus.Semantic proximity Read

The Human Cytomegalovirus Ribonucleotide Reductase Homolog UL45 Is Dispensable for Growth in Endothelial Cells, as Determined by a BAC-Cloned Clinical Isolate of Human Cytomegalovirus with Preserved Wild-Type CharacteristicsSemantic proximity Read

First continuous propagation of B19 parvovirus in a cell lineSemantic proximity Read