Article

Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

2021-06-23

Abstract excerpt

Multiplexed mRNA profiling in the spatial context provides important new information enabling basic research and clinical applications. Unfortunately, most existing spatial transcriptomics methods are limited due to either low multiplexing or assay complexity. Here, we introduce a new spatialomics technology, termed Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA), that integrates in...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 12d8f299-d15d-5965-99a0-b16ba42a681e
DOI: 10.1101/2021.06.22.449468

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysisDOI 10.1101/2021.06.22.449468Read

MERFISH 2.0, an ultra-sensitive single-cell spatial transcriptomics imaging chemistry across diverse tissue typesSemantic proximity Read

Optimizing multiplexed imaging experimental design through tissue spatial segregation estimationSemantic proximity Read

Sub-cellular Imaging of the Entire Protein-Coding Human Transcriptome (18933-plex) on FFPE Tissue Using Spatial Molecular ImagingSemantic proximity Read

Spatial Clustering Analysis with Spectral Imaging-based Single-Step Multiplex Immunofluorescence (SISS-mIF) for Assisting Histological DiagnosisSemantic proximity Read

Multiomic Spatial Imaging Assay (MSIA) – A High-plex <i>In Situ</i> Detection Method for mRNAs, Proteins and Protein-Protein Interactions using Manual And Semi-Automated WorkflowsSemantic proximity Read

Systematic Benchmarking of High-Throughput Subcellular Spatial Transcriptomics PlatformsSemantic proximity Read

Integrating single-cell RNA-seq and imaging with SCOPE-seq2Semantic proximity Read

High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodesSemantic proximity Read

Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis.Semantic proximity Read

A new spatial multi-omics approach to deeply characterize human cancer tissue using a single tissue sectionSemantic proximity Read

Automated Intelligent Microscopy for Phenotype Identification, Spatial Localization, and Retargeting of Cells.Semantic proximity Read

GPU-accelerated, self-optimizing processing for 3D multiplexed iterative RNA-FISH experimentsSemantic proximity Read