Article

Multiplex shRNA Screening of Germ Cell Development by <i>in vivo</i> Transfection of Mouse Testis

2016-03-31

Abstract excerpt

Spermatozoa are one of the few mammalian cells types that cannot be fully derived in vitro , severely limiting the application of modern genomic techniques to study germ cell biology. The current gold standard approach of characterizing single gene knockout mice is slow as generation of each mutant line can take 6-9 months. Here, we describe an in vivo approach to rapid functional screening of germline genes bas...

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Literature Corpus work: 11a76344-198a-59e1-9bf0-61fe941a39a6
DOI: 10.1101/046391

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