Article
Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing.
Molecular cell - 21 Mar 2019
Rodriguez-Meira Alba, Buck Gemma, Clark Sally-Ann, Povinelli Benjamin J, Alcolea Veronica, Louka Eleni, McGowan Simon, Hamblin Angela, Sousos Nikolaos, Barkas Nikolaos, Giustacchini Alice, Psaila Bethan, Jacobsen Sten Eirik W, Thongjuea Supat, Mead Adam J
Abstract excerpt
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for resolving transcriptional heterogeneity. However, its application to studying cancerous tissues is currently hampered by the lack of coverage across key mutation hotspots in the vast majority of cells; this lack of coverage prevents the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we...
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