Article

Highly efficient site-directed gene insertion in primary human natural killer cells using homologous recombination and CRISPaint delivered by AAV

2019-08-22

Abstract excerpt

Human peripheral blood natural killer (NK) cells have strong antitumor activity and have been used successfully in several clinical trials. Modifying NK cells with a chimeric antigen receptor (CAR) can improve their targeting and increase specificity. However, genetic modification of NK cells has been challenging due to the high expression of innate sensing mechanisms for viral nucleic acids. Recently, we describe...

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Literature Corpus work: 0cd815f9-308c-5c9d-aa2b-acbc7e14ed82
DOI: 10.1101/743377

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