Article

Tethered NOS-3, a nematode Nanos RNA-binding protein, enhances reporter expression and mRNA stability

2021-01-28

Abstract excerpt

Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein-mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C. elegans adult germline. The assay relies on a dual reporter expressing two mRNAs from a single promoter and resolved by trans-splicing. The gfp reporter 3’UTR harbors fun...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 05f53b7e-d612-54fa-a10c-754a8248749c
DOI: 10.1101/2021.01.24.428005

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Tethered NOS-3, a nematode Nanos RNA-binding protein, enhances reporter expression and mRNA stabilityDOI 10.1101/2021.01.24.428005Read

Control of the proliferation versus meiotic development decision in the C. elegans germline through regulation of GLD-1 protein accumulation.Semantic proximity Read

Recognition and long-range interactions of a minimal nanos RNA localization signal element.Semantic proximity Read

Molecular identification of smg-4, required for mRNA surveillance in C. elegans.Semantic proximity Read

Multiple RNA regulatory pathways coordinate the activity and expression pattern of a conserved germline RNA-binding proteinSemantic proximity Read

Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.Semantic proximity Read

Heterologous reporter expression in the planarian <i>Schmidtea mediterranea</i> through somatic mRNA transfectionSemantic proximity Read

<i>In vivo</i> measurements reveal a single 5’ intron is sufficient to increase protein expression level in <i>C. elegans</i>Semantic proximity Read

Repression by the 3' UTR of fem-3, a sex-determining gene, relies on a ubiquitous mog-dependent control in Caenorhabditis elegans.Semantic proximity Read

A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons.Semantic proximity Read

A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germlineSemantic proximity Read

Split-wrmScarlet and split-sfGFP: Tools for faster, easier fluorescent labeling of endogenous proteins in <i>Caenorhabditis elegans</i>Semantic proximity Read

3′ UTRs Are the Primary Regulators of Gene Expression in the C. elegans GermlineSemantic proximity Read