Article

Expansion Sequencing: Spatially Precise <i>In Situ</i> Transcriptomics in Intact Biological Systems

2020-05-15

Abstract excerpt

<h4>Abstract:</h4> Methods for highly multiplexed RNA imaging are limited in spatial resolution, and thus in their ability to localize transcripts to nanoscale and subcellular compartments. We adapt expansion microscopy, which physically expands biological specimens, for long-read untargeted and targeted in situ RNA sequencing. We applied untargeted expansion sequencing (ExSeq) to mouse brain, yielding readout o...

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Literature Corpus work: 03c41aea-4109-5e58-88fc-ca620c9f8de6
DOI: 10.1101/2020.05.13.094268

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