Article

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

2024-04-03

Abstract excerpt

Achieving effective control over microbial contamination necessitates the precise and concurrent identification of numerous pathogens. In this research, we have devised a remarkably sensitive duplex droplet digital PCR (dddPCR) reaction system to simultaneously detect Pseudomonas aeruginosa (P. aeruginosa) and Pseudomonas fragi (P. fragi). Employing comparative genomics, we identified four genes of P. fragi. By sp...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 01cbb8f9-e9a3-5843-a7f5-9a80996ab82a
DOI: 10.20944/preprints202404.0262.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in FoodsDOI 10.20944/preprints202404.0262.v1Read

Development of droplet digital PCR-based detection of bacterial pathogens in prosthetic joint infection: a preliminary study using a synthesized model plasmidSemantic proximity Read

Rapid and sensitive detection of Salmonella spp. in raw minced meat samples using droplet digital PCRSemantic proximity Read

Accurate detection of Escherichia coli O157:H7 and Salmonella enterica serovar typhimurium based on the combination of next-generation sequencing and droplet digital PCRSemantic proximity Read

Qualitative And Quantitative Detection of Surgical Pathogenic Microorganisms Escherichia Coli and Staphylococcus Aureus Based on ddPCR SystemSemantic proximity Read

A review of methods for the detection of pathogenic microorganismsSemantic proximity Read

Quantification of bacterial DNA in blood using droplet digital PCR: a pilot studySemantic proximity Read

Optimization and evaluation of a multiplex PCR assay for detection of Staphylococcus aureus and its major virulence genes for assessing food safetySemantic proximity Read

Use of Digital Droplet PCR to Detect Mycobacterium tuberculosis DNA in Whole Blood-Derived DNA Samples from Patients with Pulmonary and Extrapulmonary TuberculosisSemantic proximity Read

Aptamers against Pathogenic Bacteria: Selection Strategies and Apta-assay/Aptasensor Application for Food SafetySemantic proximity Read

One-step sensing of foodborne pathogenic bacteria using a 3D paper-based deviceSemantic proximity Read

Development of a reliable method to diagnose Streptococcus agalactiae infection by droplet digital PCRSemantic proximity Read

Direct multiplex recombinase polymerase amplification for rapid detection of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> in foodSemantic proximity Read