Preclinical evaluation of a novel multiplex ddPCR assay for detecting tetracycline resistance and differentiating Ureaplasma species

Abstract excerpt

<title>Abstract</title> <p> A multiplex ddPCR assay was developed to quantify the tet(M) and int-Tn genes and to differentiate between <italic>U. urealyticum</italic> and <italic>U. parvum</italic> . Firstly, the primers and probes for the multiplex real-time ddPCR were designed, synthesized and optimized. Next, the specificities of all the probe/primers were confirmed by BLAST analysis, and the multiplex dd...

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Literature Corpus work

2a0c4d7f-ae13-53ce-8e24-d79e4f0dcb8b

DOI

10.21203/rs.3.rs-10317377/v1