Article

Accurate detection of Escherichia coli O157:H7 and Salmonella enterica serovar typhimurium based on the combination of next-generation sequencing and droplet digital PCR

2022-10-01

Abstract excerpt

Shiga-toxin producing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium are important foodborne pathogens which are threatened to human health seriously. In this study, we firstly investigated the application and accuracy of next-generation sequencing (NGS) technology combining with droplet digital PCR (NGS + ddPCR) for the quantification of pathogenic bacteria through amplification of 16S rRNA...

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Literature Corpus work: 0c84c54e-e155-51fb-87a8-98f47c48858f
DOI: 10.1016/j.lwt.2022.113913

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