Article

Quantification of bacterial DNA in blood using droplet digital PCR: a pilot study

2022-12-02

Abstract excerpt

<h4>Aim</h4> To use genus/species-specific genes droplet digital PCR (ddPCR) assays to detect/quantify bacterial DNA from Escherichia coli , Klebsiella pneumoniae , Staphylococcus aureus and Enterococcus spp in blood samples. <h4>Methods and Results</h4> Bacterial DNA from clinical strains (4<n<12) was extracted, quantified and diluted (10-0.0001ng/μL) and ddPCR assays were performed in triplicate. These dd...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 105ce908-2e6e-5dfe-9de7-d58ef0dd2d57
DOI: 10.1101/2022.12.02.518639

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Quantification of bacterial DNA in blood using droplet digital PCR: a pilot studyDOI 10.1101/2022.12.02.518639Read

16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infectionsSemantic proximity Read

Droplet digital polymerase chain reaction for rapid broad‐spectrum detection of bloodstream infectionsSemantic proximity Read

Application of ddPCR in the rapid diagnosis of bloodstream infections: detection of Staphylococcus, Enterococcus spp, Streptococcus spp and Candida sppSemantic proximity Read

Qualitative And Quantitative Detection of Surgical Pathogenic Microorganisms Escherichia Coli and Staphylococcus Aureus Based on ddPCR SystemSemantic proximity Read

Pathogen load and species monitored by droplet digital PCR in patients with bloodstream infections: A prospective case series studySemantic proximity Read

A Comparison of Blood Pathogen Detection Among Droplet Digital PCR, Metagenomic Next-Generation Sequencing, and Blood Culture in Critically Ill Patients With Suspected Bloodstream InfectionsSemantic proximity Read

Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture- Independent ApproachSemantic proximity Read

Performance of ddPCR-GNB for microbial diagnosis of suspected bloodstream infection due to the four most common gram-negative bacteria: a prospective, multicenter studySemantic proximity Read

Clinical Evaluation of Bacterial DNA Using an Improved Droplet Digital PCR for Spontaneous Bacterial Peritonitis DiagnosisSemantic proximity Read

Development of a reliable method to diagnose Streptococcus agalactiae infection by droplet digital PCRSemantic proximity Read

Development and validation of a droplet digital PCR assay for the detection and quantification of Bartonella species within human clinical samples.Semantic proximity Read

Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistant Acinetobacter baumannii in bloodstream infectionsSemantic proximity Read