A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.

doi:10.1083/jcb.144.3.389

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Article

A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.

The Journal of cell biology - 8 Feb 1999

Hurt E, Hannus S, Schmelzl B, Lau D, Tollervey D, Simos G

Abstract excerpt

To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently...

Topics

Biological Transport, Active
Cell Nucleus
Fungal Proteins
Genetic Complementation Test
Green Fluorescent Proteins
Luminescent Proteins
Membrane Proteins
Mutation
Nuclear Pore Complex Proteins
Nuclear Proteins
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Fusion Proteins

Ribosomal Proteins

Ribosomes

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

ran GTP-Binding Protein

Source

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Identifiers

PMID: 9971735
DOI: 10.1083/jcb.144.3.389
PMCID: PMC2132911

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9971735-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.PMID 9971735Read ↗

Requirements for the nuclear export of the small ribosomal subunit.Semantic proximity Read ↗

Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p.Semantic proximity Read ↗

Factors affecting nuclear export of the 60S ribosomal subunit in vivo.Semantic proximity Read ↗

Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.Semantic proximity Read ↗

Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.Semantic proximity Read ↗

The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively.Semantic proximity Read ↗

Genetic analysis of the ribosome biogenesis factor Ltv1 of Saccharomyces cerevisiae.Semantic proximity Read ↗

The Ran GTPase cycle is required for yeast nuclear pore complex assembly.Semantic proximity Read ↗

Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.Semantic proximity Read ↗

Access to ribosomal protein Rpl25p by the signal recognition particle is required for efficient cotranslational translocation.Semantic proximity Read ↗

The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.Semantic proximity Read ↗

Rrs1p, a ribosomal protein L11-binding protein, is required for nuclear export of the 60S pre-ribosomal subunit in Saccharomyces cerevisiae.Semantic proximity Read ↗

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