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Article

A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.

The Journal of cell biology - 8 Feb 1999

Hurt E, Hannus S, Schmelzl B, Lau D, Tollervey D, Simos G

Abstract excerpt

To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently exported into the cytoplasm. In wild-type cells, GFP-labeled ribosomes are only detected by fluorescence in the...

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Identifiers

PMID: 9971735
DOI: 10.1083/jcb.144.3.389
PMCID: PMC2132911

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Research trail→

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A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.PMID 9971735Read ↗

Requirements for the nuclear export of the small ribosomal subunit.Semantic proximity Read ↗

Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p.Semantic proximity Read ↗

Factors affecting nuclear export of the 60S ribosomal subunit in vivo.Semantic proximity Read ↗

Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.Semantic proximity Read ↗

Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.Semantic proximity Read ↗

The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively.Semantic proximity Read ↗

Genetic analysis of the ribosome biogenesis factor Ltv1 of Saccharomyces cerevisiae.Semantic proximity Read ↗

The Ran GTPase cycle is required for yeast nuclear pore complex assembly.Semantic proximity Read ↗

Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.Semantic proximity Read ↗

Access to ribosomal protein Rpl25p by the signal recognition particle is required for efficient cotranslational translocation.Semantic proximity Read ↗

The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.Semantic proximity Read ↗

Rrs1p, a ribosomal protein L11-binding protein, is required for nuclear export of the 60S pre-ribosomal subunit in Saccharomyces cerevisiae.Semantic proximity Read ↗

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