Article
Molecular analysis of rheumatoid factor (RF)-negative B cell hybridomas from rheumatoid synovial tissue: evidence for an antigen-induced stimulation with selection of high mutated IgVH and low mutated IgVL/lambda genes.
Clinical and experimental immunology - 1 Jan 1999
Krenn V, König A, Hensel F, Berek C, Souto Carneiro M M, Haedicke W, Wang Y, Vollmers H, Müller-Hermelink H K
Abstract excerpt
The mutational pattern of IgVH and IgVL genes from synovial tissue B cell hybridomas (n = 8) of patients (n = 4) with rheumatoid arthritis (RA) was analysed, which had been produced by the electrofusion technique without prior in vitro stimulation. The molecular data were correlated with immunohi...
Topics
- Aged
- Amino Acid Sequence
- Antigen Presentation
- Arthritis, Rheumatoid
- B-Lymphocytes
- Base Sequence
- Enzyme-Linked Immunosorbent Assay
- Female
- Genes, Immunoglobulin
- Humans
- Hybridomas
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains